Quantitative enantioselective Raman spectroscopy

Quantitative enantioselective Raman spectroscopy.

Analytical methods for quantitative enantioselective measurements are highly desirable in the life sciences. Existing technologies have disadvantages such as limited temporal resolution, the need for molecular labeling, or high experimental complexity. To overcome these limitations, this work presents a method based on conventional Raman spectroscopy. A systematic investigation of the key param...

Universal enantioselective discrimination by Raman spectroscopy.

Distinguishing between the enantiomers of chiral substances and their quantification is an analytical challenge, in particular in the pharmaceutical and biochemical sectors. A Raman spectroscopic method for discrimination of enantiomers is proposed. Advantage is taken of the polarization properties when Raman scattering occurs in an optically active medium. It is shown that a conventional polar...

Quantitative Biological Raman Spectroscopy for Non - invasive Blood Analysis

Quantitative phase imaging and Raman micro-spectroscopy applied to Malaria

Background Malaria is due to parasitism of red blood cells (RBC) by protozoan parasites of the genus Plasmodium. Three main parameters have to be determined for patient treatment: parasite species, the rate of infected blood cells (parasitemia), and development stage. Even if a series of laboratory techniques are available, a suited treatment needs microscopy skills [1]. Microscopic observation...

Quantitative label-free RNA detection using surface-enhanced Raman spectroscopy.

Surface-Enhanced Raman Spectroscopy (SERS) was performed to detect label-free RNA. We defined conditions which make it possible to probe the four bases of RNA, in single strands of polyadenosine (pA), polyuridine (pU), polycytosine (pC) and polyguanosine (pG). We therefore present below a quantitative analysis of mixtures of non-hybridized single strands, based on the deconvolution of the SERS ...